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dc.contributor.authorBournival, Véroniquefr
dc.contributor.authorCampbell, Shirleyfr
dc.contributor.authorRoberge, Claudefr
dc.contributor.authorDoueik, Alexandrefr
dc.contributor.authorGendron, Louisfr
dc.contributor.authorPayet, Marcel Danielfr
dc.contributor.authorGallo-Payet, Nicolefr
dc.contributor.authorPraud, Jean-Paulfr
dc.date.accessioned2015-10-13T23:27:44Z
dc.date.available2015-10-13T23:27:44Z
dc.date.created2011fr
dc.date.issued2015-10-13
dc.identifierPMID:21309733fr
dc.identifier.urihttp://hdl.handle.net/11143/7961
dc.description.abstractNearly 40 potassium channels have been described in respiratory epithelial cells. Of these are found several members of the 4-transmembrane domain, 2-pore K(+) channel family (K2P family), namely Twik-1 and -2, Trek-1 and -2, Task-2, -3, and -4, Thik-1, and KCNK7. The aim of this study was to verify whether the Twik-related acid-sensitive K(+) channel, subtype 1 (Task-1) (also known as KCNK3), is present in the laryngeal mucosa in the newborn lamb. Through the use of immunohistochemistry and nested polymerase chain reaction (PCR) amplification, results indicate that Task-1 protein and mRNA are present in the laryngeal mucosa, in both the ciliated, pseudostratified columnar (respiratory) epithelium and the nonkeratinized, stratified squamous epithelium. The complete ovine Task-1 protein sequence showed high homology levels with previously reported mouse, bovine, and human Task-1 sequences. This includes a complete homology for the C-terminal amino acid sequence, which is mandatory for protein trafficking to the cell membrane. These results represent the first demonstration that Task-1, a pH-sensitive channel responsible for setting membrane potential, is present in the laryngeal mucosa of a newborn mammal.fr
dc.language.isoengfr
dc.relation.isformatofdoi:10.3109/01902148.2010.536610fr
dc.relation.ispartofISSN:0190-2148fr
dc.relation.ispartofExperimental lung researchfr
dc.subjectImmunohistochemistryfr
dc.subjectKCNK3fr
dc.subjectLambfr
dc.subjectLarynxfr
dc.subjectRespiratory epitheliumfr
dc.subjectRT-PCRfr
dc.titlePresence of task-1 channel in the laryngeal mucosa in the newborn lambfr
dc.typeArticlefr
dc.rights.holder© Taylor & Francisfr
udes.description.typestatusPost-publicationfr
udes.description.typepubRévisé et accepté par des pairsfr
udes.description.pages1-24fr
udes.description.period37(4)fr
udes.description.sponsorshipCanadian Institutes of Health Researchfr
udes.description.sponsorshipCanada Research Chair in Neonatal Respiratory Physiologyfr
udes.description.diffusionDiffusé par Savoirs UdeS, le dépôt institutionnel de l'Université de Sherbrookefr
dc.identifier.bibliographicCitationBournival, V., Desjardins, R., Campbell, S., Roberge, C., Doueik, A., Gendron, L., Payet, M. D., Gallo-Payet, N., Day, R., Praud, J.-P. (2011). Presence of task-1 channel in the laryngeal mucosa in the newborn lamb. Experimental lung Research, 37(4), 205-211. doi:10.3109/01902148.2010.536610fr
udes.description.sourceExperimental lung researchfr
udes.autorisation.depottruefr
udes.description.ordreauteursBournival, Véronique; Roberge, Claude; Campbell, Shirley; Doueik, Alexandre; Gendron, Louis; Payet, Marcel Daniel; Gallo-Payet, Nicole; Praud, Jean-Paul


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