• Français
    • English
  • Français 
    • Français
    • English
  • Login
View Document 
  •   Savoirs UdeS Home
  • Médecine et sciences de la santé
  • Publications et recherches – Médecine et sciences de la santé
  • Médecine et sciences de la santé – Articles de périodiques
  • View Document
  •   Savoirs UdeS Home
  • Médecine et sciences de la santé
  • Publications et recherches – Médecine et sciences de la santé
  • Médecine et sciences de la santé – Articles de périodiques
  • View Document
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse

All of Savoirs UdeSDomains & CollectionsBy Issue DateAuthorsTitlesSubjectsDirectorsThis CollectionBy Issue DateAuthorsTitlesSubjectsDirectors

My Account

Login

Statistics

View Usage Statistics

Genomic rearrangements in mouse C3H/10T1/2 cells transformed by X-rays, UV-C, and 3-methylcholanthrene, detected by a DNA fingerprint assay[superscript 1]

Thumbnail
View/Open
Article (1.258Mb)
Publication date
1992
Author(s)
Paquette, Benoit; Little, John B.
Editor(s)
Harvard School of Public Health. Laboratory of Radiobiology
Subject
Genomic rearrangements
 
C3H/10T1/2 cells transformed by X-rays with a DNA fingerprint essay
Show full document record
Abstract
Genomic rearrangements occurring in C3H/10T1/2 cells transformed by X-rays were examined with a DNA fingerprint assay. Four multilocus and multiallele probes were employed (M, X, H10, and H16) that detect different families of minisatellite sequences dispersed throughout the genome. Genomic rearrangements were detectable only with probe M. This specificity may be explained by a genomic instability owing to a specific sequence or structure of DNA recognized by probe M. Genomic rearrangements were detected in 5 of 12 type III foci trans formed by 600 cGy of X-rays and in all clones isolated from a previously transformed clone exposed to a second dose of 600 cGy and recloned. The latter data suggest that the stage of transformation and the occurrence of genomic rearrangement induced by X-rays may be related. An intensity shift or a complete deletion of band 2 was common to these X-ray-induced clones, as well as to clones transformed by UV-C (1 of 5) or 3-methylcholanthrene (4 of 6). This band did not hybridize to probes for the retinoblastoma gene RB or for p53. We hypothesize that the loss of band 2 may reflect a significant genetic change in the transformation of 10T1/2 cells, perhaps representing the inactivation of a tumor suppressor gene other than RB or p53. Additional rearrangements occurred in X-ray-transformed clones; these rearrangements were not observed with the other carcinogens. Aside from the changes in band 2, however, no specific pattern of genomic rearrangement was associated with X-ray transformation, and the presence or absence of rearrangements did not correlate with tumorigenicity in syngeneic nonimmunosuppressed C3H mice.
URI
http://hdl.handle.net/11143/6763
Collection
  • Médecine et sciences de la santé – Articles de périodiques [268]

DSpace software [version 5.4 XMLUI], copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
 

 


DSpace software [version 5.4 XMLUI], copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback