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  • Médecine et sciences de la santé – Articles de périodiques
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Assessment of oxidative metabolism

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Article (336.3Kb)
Publication date
2013
Author(s)
Imbeault, Emilie; Gris, Denis
Editor(s)
Gris lab of Neuroimmunology
Subject
Oxydative stress
 
Protocol
 
Oxyfative activity
 
Flow cytometry
 
Fluorescent dyes
 
Oxidative metabolism
 
Whole blood
 
Cell culture
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Abstract
Oxidative metabolism is one of the central physiological processes that regulate multiple functions in a cell including cell death and survival, proliferation, gene transcription, and protein modification. There are multitudes of techniques that are used to evaluate oxidative activity. Here, we summarize how to measure oxidative activity by flow cytometry. This versatile technique allows the evaluation of the level of oxidative activity within heterogeneous populations of cells and in cell culture. Flow cytometry is a quick method that yields highly reproducible results with small sample volumes. Therefore, it is an ideal technique for evaluating changes in oxidative activity in samples from mice.
URI
http://hdl.handle.net/11143/6711
Collection
  • Médecine et sciences de la santé – Articles de périodiques [286]

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