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dc.contributor.advisorVermette, Patrickfr
dc.contributor.authorDubois, Justinfr
dc.date.accessioned2014-05-14T19:57:01Z
dc.date.available2014-05-14T19:57:01Z
dc.date.created2011fr
dc.date.issued2011fr
dc.identifier.isbn9780494837474fr
dc.identifier.urihttp://savoirs.usherbrooke.ca/handle/11143/1641
dc.description.abstractToward the objective to create or to replace impaired tissues, it is essential to establish a culture process allowing tissue growth in vitro . The Petri dish culture or the traditional 2D culture has only a limited potential, mainly caused by a poor oxygen mass transfer to feed larger tissue constructs. In this project, an autonomous and complete bioprocess has been built to fullfill these needs. The developed reactor is versatile because many cell culture chamber designs can be connected to it. Two proportional-integral algorithms (PI) can control the dissolved oxygen concentration and the pH. The pressure, the temperature and mass flow rate are recorded in real time. An actuator allows mimicking the cardiac output flow. In this mémoire , it will be demonstrated how the reactor has been optimized to reduce the risk of bioburden. Also, the procedures to develop the control tools of the PI algorithm will be detailed. To characterize the reactor, a RTD study will be presented. To characterize the cell culture chamber, a permeability analysis using fluorescent microscopy and magnetic resonance imaging (MRI) will be exposed. Finally, two cell culture chamber designs to orient microvessel formation have been tested and these results will be presented.fr
dc.language.isoengfr
dc.publisherUniversité de Sherbrookefr
dc.rights© Justin Duboisfr
dc.subjectCellules endothélialesfr
dc.subjectOrientation de micro-vaisseaux sanguinsfr
dc.subjectChambre de culture cellulairefr
dc.subjectContrôleur PIfr
dc.subjectCoefficient de diffusion apparentfr
dc.subjectPerméabilitéfr
dc.subjectAnalyse RTDfr
dc.subjectBioréacteur à perfusionfr
dc.titleCaractérisation et optimisation d'un réacteur pour l'ingénierie tissulaire 3Dfr
dc.typeMémoirefr
tme.degree.disciplineGénie chimiquefr
tme.degree.grantorFaculté de géniefr
tme.degree.levelMaîtrisefr
tme.degree.nameM. Sc. A.fr


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