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Caractérisation et optimisation d'un réacteur pour l'ingénierie tissulaire 3D

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MR83747.pdf (5.346Mb)
Publication date
2011
Author(s)
Dubois, Justin
Subject
Cellules endothéliales
 
Orientation de micro-vaisseaux sanguins
 
Chambre de culture cellulaire
 
Contrôleur PI
 
Coefficient de diffusion apparent
 
Perméabilité
 
Analyse RTD
 
Bioréacteur à perfusion
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Abstract
Toward the objective to create or to replace impaired tissues, it is essential to establish a culture process allowing tissue growth in vitro . The Petri dish culture or the traditional 2D culture has only a limited potential, mainly caused by a poor oxygen mass transfer to feed larger tissue constructs. In this project, an autonomous and complete bioprocess has been built to fullfill these needs. The developed reactor is versatile because many cell culture chamber designs can be connected to it. Two proportional-integral algorithms (PI) can control the dissolved oxygen concentration and the pH. The pressure, the temperature and mass flow rate are recorded in real time. An actuator allows mimicking the cardiac output flow. In this mémoire , it will be demonstrated how the reactor has been optimized to reduce the risk of bioburden. Also, the procedures to develop the control tools of the PI algorithm will be detailed. To characterize the reactor, a RTD study will be presented. To characterize the cell culture chamber, a permeability analysis using fluorescent microscopy and magnetic resonance imaging (MRI) will be exposed. Finally, two cell culture chamber designs to orient microvessel formation have been tested and these results will be presented.
URI
http://savoirs.usherbrooke.ca/handle/11143/1641
Collection
  • Génie – Mémoires [1940]

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