Mechanisms of regulation of haptoglobin by C/EBPs
Ghinet, Mariana Gabriela
Effect of the proteasome inhibitor MG132 on the inflammatory response in intestinal epithelial cells. Intestinal epithelial cells participate in the acute phase response (APR) during intestinal inflammation. We have shown that acute phase protein (APP) genes are induced during the intestinal APR. One way of repressing the activity of proinflammatory molecules is by the use of different inhibitors such as proteasome inhibitors. MG132 is a highly specific inhibitor of protein degradation via the ubiquitin/proteasome system and negatively modulates the activation of specific genes involved in the intestinal acute phase response via NF-[kappa]B inhibition. Effect of C/EBP[alpha] mutant overexpression on haptoglobin expression in intestinal epithelial cells. The transcription factor C/EBP[alpha] plays an important role in the regulation of haptoglobin expression in intestinal epithelial cells, but the specific regions involved in this regulation were unknown. To determine which regions of the C/EBP[alpha] isoform are involved in the control of haptoglobin expression, we generated IEC-6 rat intestinal epithelial cell lines stably expressing different C/EBP[alpha] mutants, by infection with the retroviral vector pBabepuro."--Résumé abrégé par UMI.